Journal: Biology of Sex Differences

Article Title: Sex differences in disease severity and immune responses in murine and human inflammatory arthritis

doi: 10.1186/s13293-026-00840-w

Figure Lengend Snippet: Male CIA mice exhibit higher disease severity compared to females. Male and female CIA and saline control mice were monitored for disease severity and assigned clinical scores from day 21 after the first CII challenge until the end of experiment (day 29). (A) Line graphs representing the mean clinical scores of mice starting from day 1 to day 29. On day 29 after the first CII challenge, mice were euthanized by cardiac puncture under anesthesia for blood and serum collection and storage at −80 °C. Serum concentrations of (B) anti-mouse collagen type II antibodies (left panel) and anti-bovine collagen type II antibodies (right panel) were analyzed by ELISA. N = 5 mice per group. One of two independent experiments. Simple linear regression analysis was performed to determine the statistical difference between the lines. One-way analysis of variance (ANOVA) followed by Tukey’s multiple comparison post hoc test was used to determine the statistical significance between the groups. (*/ # p ≤ 0.05, ** p ≤ 0.005 and ***/ ### p ≤ 0.0005). In Fig. 1A, # significance of comparisons between CIA and saline control mice; *significance of comparisons between sexes of CIA mice

Article Snippet: Serum levels of mouse anti-collagen antibodies (autoantibodies) and bovine anti-collagen antibodies (antibodies to the immunizing antigen) were determined by ELISA using a Mouse Anti-mouse Type II Collagen IgG Antibody Assay Kit and Mouse Anti-Bovine Type II Collagen IgG Antibody Assay Kit, respectively, according to the manufacture’s protocol (Chondrex Inc. WA, USA).

Techniques: Saline, Control, Enzyme-linked Immunosorbent Assay, Comparison

Journal: NPJ Science of Food

Article Title: Haematococcus pluvialis ameliorates renal fibrosis by restoring mitophagy via PINK1-Parkin-p62-LC3 signaling

doi: 10.1038/s41538-025-00654-x

Figure Lengend Snippet: A , B Histopathological evaluation of renal cortical tissues through HE, Masson, and PAS staining (scale bar: 20 μm). Tubular regions (A, 63×) and glomerular structures (B, 40×). C Quantitative analysis of collagen fiber deposition (blue) in tubular regions via Masson staining using ImageJ ( n = 8). D Glomerular collagen content quantified by Masson staining and glomerular MMI measured by PAS staining ( n = 8), with magenta indicating glycogen deposition. E Blue: nuclei; Brown: FN1 and COL4A. Positive expression of FN1 and COL4A proteins in renal cortical tissues was detected by IHC staining (magnification: 63×, scale bar: 20 μm). F Quantitative analysis of positive areas (brown) in IHC results using ImageJ ( n = 8). G Western blot analysis and gray value quantification of α-SMA, FN1, and COL4A expression in renal cortical tissues from different experimental groups. β-Actin and β-Tubulin was used as a loading control. Gray value quantification of Western blotting results using ImageJ ( n = 3). H Functional enrichment analysis of FN1 gene and COL4A2 gene via RNA-seq profiling. * p < 0.05 vs. Sham group, # p < 0.05 vs. UUO group.

Article Snippet: LC3 Polyclonal antibody (14600-1-AP), p62 Polyclonal antibody (80294-1-RR), PINK1 Polyclonal antibody (23274-1-AP), Parkin Polyclonal antibody (14060-1-AP), E-cadherin Polyclonal antibody (20874-1-AP), N-cadherin Polyclonal antibody (No.: 22018-1-AP), Vimentin Polyclonal antibody (10366-1-AP), COL4A2 Polyclonal antibody (55131-1-AP), FN1 Monoclonal antibody (66042-1-Ig), β-Actin Polyclonal antibody (20536-1-AP), and α-Tubulin Polyclonal antibody (11224-AP) were from Proteintech group Co., Ltd (Wuhan, China).

Techniques: Staining, Expressing, Immunohistochemistry, Western Blot, Control, Functional Assay, RNA Sequencing

Journal: Current Protocols

Article Title: Cryopreservation of Human Adult Ventricular Tissue for the Preparation of Viable Myocardial Slices

doi: 10.1002/cpz1.70068

Figure Lengend Snippet: Primary Antibodies

Article Snippet: Type III Collagen , 1:2000 , Southern Biotech , 1330‐01.

Techniques:

Journal: Current Protocols

Article Title: Cryopreservation of Human Adult Ventricular Tissue for the Preparation of Viable Myocardial Slices

doi: 10.1002/cpz1.70068

Figure Lengend Snippet: Extracellular matrix. Representative images of immunohistochemical analysis of fresh and cryopreserved cardiac tissue. Type I Collagen (Collagen‐I) is shown in orange (A) and Type III Collagen (Collagen‐III) is shown in yellow (B) . Counterstaining of nuclei was performed with DAPI (blue).

Article Snippet: Type III Collagen , 1:2000 , Southern Biotech , 1330‐01.

Techniques: Immunohistochemical staining